Fast and Efficient Lysis
The buffer incorporates two separate lysis mechanisms to maximize lysis of a wide variety of organisms. A chemical process disrupts the lipid bilayer, while enzymes simultaneously digest membrane proteins. As lysis proceeds immediately, the buffer can be used to inactivate potentially infectious agents at the time of sample collection.
Maximize DNA/RNA Recovery
RNA is notoriously susceptible to degradation by RNase, making it difficult to work with. As the buffer’s specialized enzymes are active at ambient temperatures, DNase, RNase, and antimicrobial proteins are immediately digested as cells lyse, protecting DNA and RNA from degradation and increasing extraction efficiency.
Lyse Liquid or Solid Samples
The Enzymatic DNA/RNA Extraction Buffer is available in two formats. The 1X concentration allows solid samples such as swabs, cell pellets, and membrane filters to be placed into buffer for lysis.
The 10X concentration can be added directly to liquid samples such as water, biological fluids, or transport media. This minimizes dilution of the sample, allowing sensitive detection by PCR.
Simple Protocol, Direct PCR
There’s no need for any centrifugation or mechanical agitation steps for most samples. Simply expose cells to lysis buffer for 15 minutes at ambient temperature, then incubate for 5 minutes at 98 ºC to inactivate enzymes. The buffer is safe to handle; all reagents are non-toxic.
The Enzymatic DNA/RNA Extraction Buffer displays low PCR inhibition, allowing larger volumes of lysate to be added to PCR reactions for more sensitive detection. With our inhibition-tolerant PCR Master Mixes, up to 25% lysate may be used depending on sample type.
The Enzymatic DNA/RNA Extraction Buffer has been tested to work with: Bacteria (gram-positive, gram-negative), Human cells (buccal, epithelial, blood), Animal cells, Viruses (RNA and DNA)
The buffer degrades DNase/RNase and stabilizes nucleic acids chemically, so extracts are extremely stable short-term, even at ambient temperatures.
Both DNA and RNA extracts may be stored for up to 72 hours at 25 ºC, allowing flexibility in sample processing and transport. For long-term storage store DNA extracts at -20 ºC, and RNA extracts at -70 ºC.