There’s no need for any heating, centrifugation, or mechanical agitation steps for most samples. Simply expose cells to lysis buffer for 10 minutes at ambient temperature. Extraction efficiency for difficult-to-lyse cells can be improved by vortexing or homogenization.
The buffer is safe to handle; all reagents are non-toxic.
Lyse Liquid or Dry Samples
The One-Step DNA/RNA Extraction Buffer is available in two formats. The 1X concentration allows solid samples such as swabs, cell pellets, and membrane filters to be placed into buffer for lysis.
The 10X concentration can be added directly to liquid samples such as water, biological fluids, or transport media. This minimizes dilution of the sample, allowing sensitive detection by PCR.
Direct, Sensitive PCR
The One-Step DNA/RNA Extraction Buffer displays low PCR inhibition, allowing larger volumes of lysate to be added to PCR reactions for more sensitive detection. With our inhibition-tolerant PCR Master Mixes, up to 25% lysate may be used depending on sample type.
The One-Step DNA/RNA Extraction Buffer has been tested to work with:
Bacteria (gram-positive, gram-negative), Human cells (buccal, epithelial), Animal cells, Viruses (RNA and DNA)
DNA is a fairly stable molecule, but RNA notoriously degrades quickly. At 25 ºC, the One-Step DNA/RNA Extraction Buffer stabilizes DNA for up to 72 hours, and RNA for up to 24 hours, allowing flexibility in sample processing. DNA extracts can be stored long-term at -20 ºC, while RNA extracts can be stored at -70 ºC.
The buffer does not protect nucleic acid from DNase or RNase activity. For samples where this is a concern or for sensitive RNA extraction, we recommend our Enzymatic DNA/RNA Extraction Buffer.