Description
Sahara Multiplex qPCR Master Mix is a 2X Taq mix for probe-based Real-Time PCR. It quantitatively amplifies singleplex and multiplex (up to 4-plex) qPCR targets at high efficiency from purified samples and crude extracts.
The Most Stable Master Mix on the Planet
Stable at 50 ºC for 8 days, or 25 ºC for 3 months, Sahara mixes ship economically at ambient temperatures, prevent Cq drift over time due to thermal degradation, and are ideal for high throughput or field applications where temperature control is not always ideal.
Amplifies Everything Without Inhibition
Sahara Multiplex qPCR Master Mix has been validated against genomic, plasmid, and cDNA targets from a wide range of organisms. The mix supports both standard and fast protocols, and amplifies GC-rich targets up to 70%.
Numerous enhancers bind & inactivate PCR inhibitors, allowing amplification from crude extracts of:
- Blood
- Food & beverages
- Plant & animal tissue
- Environmental air & water samples
Multiplex with Confidence
Multiplex qPCR often suffers from competitive inhibition, where a high copy number target amplifies early, consuming reactants that prevent lower copy number targets from amplifying. With high levels of Taq, dNTPs, and multiplexing enhancers, Sahara Multiplex has been demonstrated to amplify four multiplex targets simultaneously, with no change in Cq when compared with singleplex reactions.
Simplify your PCR
Set up reactions with ease at room temperature: an aptamer-based hotstart system prevents amplification from occurring below 50 ºC, while a blue non-interfering loading dye helps avoid pipetting errors. When activated by the addition of UNG, a dUTP carry-over prevention system prevents false positives from amplicon contamination.