Chai Green qPCR Master Mix - Gateway Biosciences

Chai Green qPCR Master Mix

Get clear melt curves and High Resolution Melt (HRM) results with the highly fluorescent Chai Green dye. Highly efficient mix resists inhibition, even in environmental samples. For dye-based qPCR.

  • Produce excellent melt curves & HRM results with the highly fluorescent Chai Green dye
  • Overcomes inhibition in crude extracts and environmental samples
  • Universal mix runs fast protocols, amplifies GC-rich targets, and incorporates aptamer-based hot start and dUTP carry-over prevention systems
  • License-free for commercial or diagnostic use
  • Cat#: R02201S-SE, 2 x 1 mL
  • Cat#: R02201L-SE, 2 x 5 mL
  • Cat#: R02201M-SE, 5 x 1 mL
  • Cat#: R02201B-SE, 1 x 50 mL


Chai Green qPCR Master Mix is a 2X Taq mix for dye-based Real-Time PCR, melt curve analysis, and High Resolution Melt (HRM). It quantitatively amplifies singleplex and duplex qPCR targets at high efficiency from purified samples and crude extracts.

Better Melt Curves & HRM

As Chai Green is less inhibitory to PCR than SYBR Green, it can be used at significantly higher concentrations. The resulting increased fluorescence improves the ability to differentiate small differences via melt and HRM analysis.

Amplifies Everything Without Inhibition

Chai Green qPCR Master Mix has been validated against genomic, plasmid, and cDNA targets from a wide range of organisms. The mix supports both standard and fast protocols, and amplifies GC-rich targets up to 70%.

Thanks to numerous enhancers which bind & inactivate inhibitors, the mix resists inhibition in crude extracts from:
• Food & beverages
• Plant & animal tissue
• Environmental air & water samples

Simplify your PCR

Set up reactions with ease at room temperature: an aptamer-based hotstart system prevents amplification from occurring below 50 ºC, while a visible loading dye helps avoid pipetting errors. When activated by the addition of UNG, a dUTP carry-over prevention system prevents false positives from amplicon contamination.